Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (8B.2C12), APC, eBioscience

Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (8B.2C12), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC-eFluor 780
• Biotin
• eFluor 450
• Functional Grade
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 8B.2C12 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. CD366 is a type I transmembrane protein containing an immunoglobulin and mucin-like domain in its extracellular region and a tyrosine phosphorylation motif in its cytoplasmic region. It is selectively expressed by differentiated CD4+ Th1 and CD8+ Tc1 cells, but absent on Th2 and Tc2 cells. Other hematopoietic cell types, including naive T cells, B cells, macrophages, and dendritic cells, do not express CD366 at the protein level.

Expression of CD366 increases at a late stage of Th1 differentiation, suggesting a role in transport or effector function rather than differentiation. In an EAE model, CD366 was expressed on most CD4+ and CD8+ T cells in the CNS during disease onset, while peripheral expression remained low. Administration of 8B.2C12 in vivo resulted in a hyperacute and atypical disease phenotype, suggesting CD366 engagement may promote macrophage activation and autoimmune severity. The Tim gene family may play an important role in regulating autoimmunity and allergies.

The 8B.2C12 antibody binds strongly to the BALB/c allele of CD366, with weaker reactivity to the C57Bl/6 allele.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.06 µg/test (100 µL final volume).
Laser: Red Laser (Excitation 633–647 nm; Emission 660 nm)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2) is a 281 amino acid Type-1 Th1-specific cell surface glycoprotein expressed on terminally differentiated CD4+ Th1 and CD8+ Tc1 cells. It consists of an IgV-like domain, a mucin-like domain, and a conserved tyrosine phosphorylation motif. TIM3 is involved in macrophage activation and autoimmune disease induction, downregulating aggressive Th1-mediated responses and promoting immune tolerance. It is implicated in Experimental Autoimmune Encephalomyelitis (EAE), enhancing disease severity in mice.

For Research Use Only. Not for use in diagnostic procedures.

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