Thermo Fisher Scientific CD247 (CD3 zeta) Monoclonal Antibody (6B10.2), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats:

• APC
• PE
• Request custom conjugation

Product Specific Information

The 6B10.2 monoclonal antibody reacts with human CD247 (CD3 zeta chain). The CD3 complex consists of gamma, delta, epsilon, and zeta chains, forming part of the TCR/CD3 complex. CD3 zeta is expressed on immature and mature thymocytes, T lymphocytes, and NK cells. It plays a key role in T cell activation via tyrosine phosphorylation of ITAM motifs.

This antibody recognizes a transmembrane epitope of the CD3 zeta chain; therefore, intracellular staining is required for flow cytometric analysis. It detects the zeta chain as a homodimer but not as a monomer.

Applications Reported:
Intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-titrated and tested using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00) following BestProtocols® Protocol A.
Use 5 µL (0.03 µg) per test in 100 µL volume, with 10⁵–10⁸ cells/test.

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm, excited by the 488 nm blue laser. Recommended filter: 710/50 bandpass (695/40 acceptable). Protect from light due to photo-induced oxidation sensitivity.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.

Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD3z complex is essential for transducing antigen recognition signals into T cells and regulating TCR complex expression. CD3 subunits (gamma, delta, epsilon, zeta) are encoded on chromosome 11 and contain ITAM motifs for signal transduction. CD3 is expressed on most T lymphocytes and thymocytes, but not on B or NK cells. Altered CD3 expression is associated with immune deficiencies such as Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency due to CD3δ/ε/ζ defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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