Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Alexa Fluor 700, eBioscience
마우스 CD170(Siglec F)을 인식하는 1RNM44N 단클론 항체로, Alexa Fluor 700 형광체가 결합된 제품입니다. 유세포 분석(Flow Cytometry)에 적합하며, 0.125 µg/test로 사용 가능합니다. 4°C 암소 보관, 포름알데히드 고정 시에도 인식 가능한 에피토프를 타겟합니다.
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Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Alexa Fluor 700, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 10 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1RNM44N |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637126 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Brilliant UV 395 / 563 / 615 / 737
- Brilliant Violet 480 / 605 / 711
- eFluor 660
- NovaFluor Blue 610-70S / 660-120S / 800
- NovaFluor Red 685 / 710
- NovaFluor Yellow 690 / 810
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 780
- Custom conjugation available upon request
Product Specific Information
This 1RNM44N monoclonal antibody recognizes mouse CD170 (Siglec F), a cell surface lectin in the Ig superfamily that binds sialic acid-containing glycoconjugates. The cytoplasmic domain contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) initiating intracellular signaling upon ligand binding.
Siglec F is primarily expressed on eosinophils and alveolar macrophages, with lower levels on immature myelomonocytic cells. It is the functional ortholog of human Siglec 8 but is not expressed on mast cells in mice.
This antibody recognizes a formaldehyde-fixed epitope.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse thioglycolate-elicited peritoneal exudate cells at ≤0.125 µg/test (100 µL final volume). Cell numbers can range from 10⁵–10⁸ cells/test. Titrate for optimal performance.
Fluorochrome Information:
Alexa Fluor™ 700 emits at 723 nm, excited by the red laser (633 nm). Recommended optical setup: 685 LP mirror and 710/20 filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red Laser.
Post-manufacturing filtration: 0.2 µm.
Target Information
SIGLEC5 is a putative adhesion molecule mediating sialic acid-dependent binding. It interacts with both α2,3- and α2,6-linked sialic acids, possibly masked by cis interactions on the same cell surface. Expressed in monocytic/myeloid lineage cells, SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, and bone marrow, with lower expression in lymph node, lung, appendix, placenta, pancreas, and thymus. It is expressed in monocytes and neutrophils but absent in early leukemic myelomonocytic cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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