Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), APC, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), APC, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC-eFluor 780
• Biotin
• Brilliant UV/Violet/Yellow series
• eFluor 450, 506, 660
• FITC
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436–780
• NovaFluor Blue/Red/Violet/Yellow series
• Functional Grade
• Custom conjugation available

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds to MHC class II molecules on antigen-presenting cells and plays a critical role in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse thymocytes and splenocytes (≤0.125 µg/test, 100 µL final volume). Optimal titration recommended.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. It is expressed primarily on T helper cells, and also on monocytes, macrophages, and dendritic cells. Tissue-level expression includes thymus, lymph nodes, tonsils, spleen, and some non-lymphoid tissues such as brain and gut. CD4 participates in early T-cell activation via association with the T-cell receptor complex and Lck kinase, and may mediate neuronal damage in infectious or immune-related CNS diseases. Multiple spliced transcripts are known (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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