Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660, 700
• APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor 450, 506, 570, 615
• FITC
• NovaFluor Blue & Yellow series
• Pacific Orange
• PE, PE-Cyanine5/5.5/7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series (436–780)
• Request custom conjugation

Product Specific Information

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays a critical role in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck. Binding of RM4-5 is blocked by GK1.5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse thymocyte or splenocyte suspensions at ≤0.125 µg/test
  • A test = antibody amount for 100 µL final volume
  • Cell number: 10⁵–10⁸ cells/test (empirical determination recommended)
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD4 antigen is involved in recognition of MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed in T helper cells, and also in monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues such as brain and gut. CD4 participates in early T-cell activation via association with the T-cell receptor complex and tyrosine kinase Lck, and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts exist (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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