Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), APC, eBioscience
Thermo Fisher Scientific의 CD3 Monoclonal Antibody (17A2), APC 형은 마우스 CD3 복합체를 인식하는 랫 IgG2b 항체로, 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 0.5 µg/test로 사용 가능하며, T세포 활성화 연구에 적합합니다. 651/660 nm APC 형광 특성 보유.
- 카탈로그번호
- 17-0032-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), APC, eBioscience
Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), APC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 117 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 17A2 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10598661 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 700
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 737 / 805
- Brilliant Violet 421 / 480 / 650
- eFluor 450 / 506 / 660
- FITC
- Functional Grade
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta, and epsilon are required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates intracellular signaling pathways resulting in cellular activation and proliferation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.5 µg/test (100 µL final volume).
Cell number can range from 10⁵ to 10⁸ cells/test. Antibody titration recommended for optimal performance.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD3 subunit complex is crucial for transducing antigen-recognition signals into T cells and regulating surface expression of the TCR complex. CD3 subunits (gamma, delta, epsilon, zeta) are members of the immunoglobulin superfamily encoded by genes on human chromosome 11. They associate with cytoplasmic signal transduction molecules via tyrosine-based motifs. CD3 is expressed on most T lymphocytes and thymocytes but not on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.