Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), APC, eBioscience
인간 CD3 복합체의 엡실론 서브유닛을 인식하는 단클론 항체로, APC 형광체로 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, T 세포 활성화 및 면역반응 연구에 적합합니다. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 17-0037-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 39 publications |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Nematode |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT3 |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1907373 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 605
- eFluor 450 / 506 / 660
- FITC
- Functional Grade
- NovaFluor Blue 510 / 585
- NovaFluor Yellow 590 / 660 / 730
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
Description:
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has potent immunosuppressive properties in vivo and has been used effectively in the treatment of renal, heart, and liver allograft rejection. CD3 subunits (gamma, delta, epsilon) are required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates intracellular signaling leading to activation and proliferation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated for flow cytometry of normal human peripheral blood cells. Use 5 µL (0.06 µg) per test (100 µL final volume). Cell number can range from 10⁵ to 10⁸ cells/test.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and regulating surface expression of the TCR complex. T cell activation involves CD3 gamma, delta, epsilon, and zeta subunits, which are structurally related members of the immunoglobulin superfamily encoded on chromosome 11. These subunits associate with cytoplasmic signal transduction molecules via double tyrosine-based motifs. CD3 may play roles in TCR-induced growth arrest, cell survival, and proliferation.
CD3 antigen is expressed on:
- 68–82% of normal peripheral blood lymphocytes
- 65–85% of thymocytes
- Purkinje cells in the cerebellum
It is not expressed on B or NK cells. Decreased T lymphocyte percentages may occur in autoimmune diseases. Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.