Thermo Fisher Scientific CD5 Monoclonal Antibody (HIS47), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
The HIS47 monoclonal antibody reacts with rat CD5, a 67 kDa surface protein expressed by most thymocytes and mature T cells. Unlike mouse and human CD5, the CD5⁺ B cell subset has not been identified in rats. CD5 acts as a counter-receptor for CD72 and plays a role in T-B cell interactions.

Applications Reported:
This HIS47 antibody is reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of rat splenocytes. Recommended usage is ≤ 0.25 µg per test (100 µL final volume). Cell number can range from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.

Optical Properties:

• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD5 is a 67 kDa single-chain transmembrane glycoprotein expressed on human T-lymphocytes, thymocytes, and subsets of activated B cells. It is involved in regulating T-cell proliferation and is associated with various T-cell leukemias and lymphomas. CD5 expression is high in thymocytes (95%) and peripheral blood lymphocytes (72%), with main reactivity in T cell areas of lymph nodes. Diseases linked to CD5 dysfunction include thymus cancer and Richter’s Syndrome.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

제품 이미지