Thermo Fisher Scientific CD5 Monoclonal Antibody (HIS47), APC, eBioscience
Rat CD5 단백질을 인식하는 HIS47 단클론 항체로, APC로 결합되어 유세포 분석(Flow Cytometry)에 적합합니다. 0.25 µg/test 농도로 사용 가능하며, 651/660 nm 파장에서 Excitation/Emission 특성을 가집니다. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 17-0050-82
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIS47 |
| Conjugate | APC |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469329 |
Product Specific Information
Description:
The HIS47 monoclonal antibody reacts with rat CD5, a 67 kDa surface protein expressed by most thymocytes and mature T cells. Unlike mouse and human CD5, the CD5⁺ B cell subset has not been identified in rats. CD5 acts as a counter-receptor for CD72 and plays a role in T-B cell interactions.
Applications Reported:
This HIS47 antibody is reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of rat splenocytes. Recommended usage is ≤ 0.25 µg per test (100 µL final volume). Cell number can range from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.
Optical Properties:
- Excitation: 633–647 nm
- Emission: 660 nm
- Laser: Red Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD5 is a 67 kDa single-chain transmembrane glycoprotein expressed on human T-lymphocytes, thymocytes, and subsets of activated B cells. It is involved in regulating T-cell proliferation and is associated with various T-cell leukemias and lymphomas. CD5 expression is high in thymocytes (95%) and peripheral blood lymphocytes (72%), with main reactivity in T cell areas of lymph nodes. Diseases linked to CD5 dysfunction include thymus cancer and Richter’s Syndrome.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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