Thermo Fisher Scientific WTAP Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).

Target Information

Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects approximately 1 in 10,000 infants and occurs in both sporadic and inherited forms.
The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn²⁺ finger transcriptional activators, and functions as a transcriptional repressor.

WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may regulate mRNA splicing. It is found in nuclear speckles, where it controls the G2/M cell cycle transition by binding to the 3′ UTR of cyclin A2, enhancing its stability.
Additionally, WTAP inhibits expression of WT1 target genes and can impair WT1’s DNA-binding ability. Two isoforms of WTAP exist due to alternative splicing.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.