Thermo Fisher Scientific TdT Polyclonal Antibody
Rabbit polyclonal antibody recognizing human TdT protein. Suitable for Western blot and IHC (paraffin) applications. Recommended for thymus tissue as positive control. For research use only, not for diagnostic procedures.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide in the internal region of human TdT protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550075 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is thymus tissue.
Target Information
Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase located in the cell nucleus that catalyzes the polymerization of deoxynucleotides at the 3′ hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template.
TdT is considered a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leukemias. The determination of TdT expression is especially valuable for differentiating histologically between lymphoblastic lymphoma and Burkitt’s lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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