Thermo Fisher Scientific Thymidylate Synthase Polyclonal Antibody

Applications

• Immunohistochemistry (Paraffin) [IHC (P)]: Tested dilution 1:100

Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is colon carcinoma.

Target Information

Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis.
TS is a critical target for fluoropyrimidines, an important group of antineoplastic drugs widely used in the treatment of solid tumors.
Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP, which inactivates TS by forming a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate.
Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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