Thermo Fisher Scientific CD15 Monoclonal Antibody (HI98), eBioscience
인간 CD15(Lewis X)를 인식하는 HI98 단클론 항체로, 유세포분석(Flow Cytometry) 및 면역조직화학(IHC) 등에 사용 가능. 고순도(>90%)로 정제되었으며, 다양한 형광 및 효소 표지 형식 제공. 연구용으로만 사용.
- 카탈로그번호
- 14-0159-xx (3개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD15 Monoclonal Antibody (HI98), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | - |
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (Paraffin) (IHC-P) | 10 µg/mL |
| Immunohistochemistry (Frozen) (IHC-F) | 10 µg/mL |
| Immunocytochemistry (ICC/IF) | - |
| Flow Cytometry (Flow) | 0.25 µg/test |
| In vitro Assay (IV) | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HI98 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864832 |
Available Conjugates
- APC
- Biotin
- eFluor 450
- eFluor 615
- FITC
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The HI98 monoclonal antibody reacts with human CD15 (Lewis X), a 3-fucosyl-N-acetyllactosamine carbohydrate moiety expressed by granulocytes. Monocytes express this structure at varying degrees, while lymphocytes are negative.
Applications Reported:
HI98 antibody is suitable for flow cytometric analysis and immunohistochemical staining of frozen and FFPE tissue sections (IHC-F, IHC-P).
Applications Tested:
- Flow cytometry: ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
- Immunohistochemistry (FFPE): ≤10 µg/mL.
Antibody should be titrated for optimal assay performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types including granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and tumor cells.
Variants such as sialyl-Lewis X and sulphated Lewis X exist. High surface expression of Le(x) promotes calcium-dependent self-aggregation, involved in processes like embryo compaction and teratocarcinoma cell autoaggregation. Sialyl-Le(x) and sialyl-Le(a) serve as selectin ligands. CD15 expression is widely used in the diagnosis of Hodgkin’s disease.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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