Thermo Fisher Scientific CD15 Monoclonal Antibody (HI98), eBioscience

Thermo Fisher Scientific CD15 Monoclonal Antibody (HI98), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugates

• APC
• Biotin
• eFluor 450
• eFluor 615
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

The HI98 monoclonal antibody reacts with human CD15 (Lewis X), a 3-fucosyl-N-acetyllactosamine carbohydrate moiety expressed by granulocytes. Monocytes express this structure at varying degrees, while lymphocytes are negative.

Applications Reported:
HI98 antibody is suitable for flow cytometric analysis and immunohistochemical staining of frozen and FFPE tissue sections (IHC-F, IHC-P).

Applications Tested:

• Flow cytometry: ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
• Immunohistochemistry (FFPE): ≤10 µg/mL.
  Antibody should be titrated for optimal assay performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types including granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and tumor cells.

Variants such as sialyl-Lewis X and sulphated Lewis X exist. High surface expression of Le(x) promotes calcium-dependent self-aggregation, involved in processes like embryo compaction and teratocarcinoma cell autoaggregation. Sialyl-Le(x) and sialyl-Le(a) serve as selectin ligands. CD15 expression is widely used in the diagnosis of Hodgkin’s disease.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.