Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), eBioscience
인간 CD14 항원을 인식하는 61D3 클론의 마우스 단일클론 항체로, 유세포분석(Flow), 면역조직화학(IHC), 기능적 분석 등에 적합합니다. 고순도(>90%)로 정제된 액상 형태이며 다양한 형광 및 기능성 접합체 옵션 제공.
- 카탈로그번호
- 14-0149-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), eBioscience
Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 20 µg/mL |
| Immunocytochemistry (ICC/IF) | - |
| Flow Cytometry (Flow) | 0.5 µg/test |
| Neutralization (Neu) | - |
| Functional Assay | Assay-Dependent |
| Miscellaneous PubMed (Misc) | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 61D3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467129 |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue 510, 555, 585, 610-30S, 660-40S, 800
- NovaFluor Yellow 570, 660
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, interfollicular macrophages, and some dendritic cells. CD14 serves as a receptor for LPS-LBP complexes, binding with high affinity to monocytes.
Applications Reported:
- Flow cytometric analysis
- Immunohistochemical staining of formalin-fixed paraffin-embedded human tissue
- In vitro functional studies (functional grade recommended for assays)
Applications Tested:
- Flow cytometry: ≤ 0.5 µg/test (100 µL sample volume, 10⁵–10⁸ cells/test)
- IHC (Paraffin): ≤ 20 µg/mL (low pH antigen retrieval recommended)
Antibody should be titrated for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein expressed on monocytes, macrophages, and neutrophils. It functions as a lipopolysaccharide receptor and is released into serum as soluble CD14 (sCD14). CD14 interacts with LPS-binding protein (LBP) to mediate immune responses and plays roles in inflammation, infection, and allergy. Alternative splicing yields multiple transcript variants encoding the same isoform. Associated diseases include Mycobacterium chelonae infection and croup.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.