Thermo Fisher Scientific Human/Mouse/Rat Thymosin-beta4 Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

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Miscellaneous PubMed (Misc)

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View 1 publication 1 publication

Product Specifications

Species

Human, Mouse, Rat

Published species

Mouse

Expression System

E. coli

Amino acid sequence

RMSDKPDMAE IEKFDKSKLK KTETQEKNPL PSKETIEQEK QAGES

Molecular weight

5.2 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Pretreatment of primary lung fibroblasts with recombinant Thymosin-beta4, using a concentration of 0.5 - 10 ug/ml, produces a protective effect against hydrogen peroxide induced cell death.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Product Specific Information

140-14-1MG will be provided as 2 x 500 µg (140-14-500UG).

Recombinant Human/Murine/Rat Thymosin-beta4 is a 5.2 kDa glycoprotein containing 45 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Thymosin-beta4 is a small, actin-sequestering protein belonging to the thymosin-beta family that is found at high concentrations within the spleen, thymus, and peritoneal macrophages, where it is most notably responsible for the organization of cytoskeletal structure. In mammalian tissues, this protein acts as a modulator for the polymerization/depolymerization of actin through the formation of a 1:1 complex with the monomer G (globular)-actin, and inhibits actin`s polymerization to form F (filamentous) actin, which together with other proteins binds microfilaments to construct the cytoskeleton. Commonly found at significant quantities within the brain, lungs, liver, kidneys, testes, and heart, thymosin-beta4 has also been shown to be synthesized by cells unrelated to the reticuloendothelial system, such as myoblasts and fibroblasts, and expressed at irregular levels by several hemopoietic cell lines, malignant lymphoid cells and myeloma cells. In addition to regulating actin polymerization, research has also found thymosin-beta4 to stimulate the secretion of hypothalamic luteinizing hormone-releasing hormone and luteinizing hormone, inhibit the migration of peritoneal macrophages, induce phenotypic changes in T cell lines during early host defense mechanisms, and inhibit the progression of hematopoietic pluripotent stem cells into the S-phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.