
Thermo Fisher Scientific CCL3 Polyclonal Antibody
Human CCL3/MIP-1α를 인식하는 Goat Polyclonal Antibody로, WB, IHC, ELISA, Neutralization 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 인간 시료에 높은 특이성과 낮은 교차 반응성을 보임.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL | - |
| ELISA | 0.1–0.4 µg/mL (Detection), 0.2–0.8 µg/mL (Capture) | - |
| Neutralization (Neu) | 2–8 µg/mL | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant human CCL3/MIP-1 alpha (Ala27–Ala92) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609538 |
Product Specific Information
- In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human MIP-1 beta, MIP-1 gamma, MIP-3 alpha, MIP-3 beta, and recombinant mouse MIP-1 alpha.
- Neutralizes the biological activity of recombinant human CCL3/MIP-1 alpha.
- In chemotaxis assays, partially neutralizes rhMIP-1 beta at 20-fold higher IgG concentration.
- Does not neutralize rmMIP-1 beta or rmMIP-1 alpha.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level: <0.10 EU per 1 µg of antibody (LAL method).
Target Information
MIP-1α and MIP-1β are CC chemokines involved in immune response to bacterial, viral, parasitic, and fungal infections. They regulate trafficking and activation of inflammatory cells such as macrophages, lymphocytes, and NK cells.
MIP-1α selectively attracts CD8⁺ lymphocytes, while MIP-1β attracts CD4⁺ lymphocytes. Both are potent chemoattractants for B cells, eosinophils, and dendritic cells, active on both human and murine hematopoietic cells.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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