Thermo Fisher Scientific 53BP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:1,000

View 2 publications 2 publications

Flow Cytometry (Flow)

2-5 µg/million cells

-

ChIP assay (ChIP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Bat, Bovine, Dog, Human, Mouse, Rodent, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residues 1925 and the C-terminus (residue 1972) of human 53BP1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:53BP1,uniProtId:Q12888-1,ncbiNodeId:9606,antigenRange:1925-1972,antigenLength:1972,antigenImageFileName:PA1-16566_53BP1_Q12888-1_Rabbit.svg,antigenImageFileNamePDP:PA1-16566_53BP1_Q12888-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2303935

Product Specific Information

Human samples have been tested in Western Blot and ICC/IF, and mouse samples have been tested in ICC/IF only.

Suggested positive control: U205, 293T, and MO59K cell lysates and MEF lysates .

Target Information

53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.