Thermo Fisher Scientific 53BP1 Polyclonal Antibody
다양한 종(인간, 마우스, 랫 등)에 반응하는 Rabbit Polyclonal 53BP1 항체. Western blot, IHC, ICC/IF, Flow, ChIP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공. DNA 손상 반응 및 종양 억제 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:500 | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:1,000 | View 2 publications |
| Flow Cytometry (Flow) | 2–5 µg/million cells | - |
| ChIP assay (ChIP) | Assay-Dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bat, Bovine, Dog, Human, Mouse, Rodent, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 1925 and C-terminus (residue 1972) of human 53BP1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2303935 |
Product Specific Information
- Human samples tested in Western Blot and ICC/IF
- Mouse samples tested in ICC/IF
- Suggested positive controls: U205, 293T, MO59K, and MEF cell lysates
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to sites of double-strand breaks. It may function in checkpoint signaling during mitosis, enhance TP53-mediated transcriptional activation, and participate in DNA repair by maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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