Thermo Fisher Scientific FANCD2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

2.5-5 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Flow Cytometry (Flow)

2-5 µg/mL

-

Immunoprecipitation (IP)

1:10-1:500

-

ChIP assay (ChIP)

Assay-Dependent

-

Inhibition Assays (IA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Avian, Dog, Human, Marsupial, Mouse, Non-human primate, Rat, Zebrafish

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2102037

Product Specific Information

In Western blot, this antibody should recognize a band at ~166 kDa (post-translationally modified form).

Additional bands may be seen at lower molecular weights.

In immunofluorescence, this has been tested in human MMC and IR-treated MEF cells.Suggested positive control: Hela whole cell extract.

Target Information

FANCD2 (Fanconi anemia group D2 protein) is required for maintenance of chromosomal stability. It promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-stranded breaks, both by homologous recombination and single-stranded annealing. It is required for the targeting/stabilization of BLM to non-centromeric abnormal structures induced by replicative stress. FANCD2 promotes BRCA2/FANCD1 loading onto damaged chromatin. Defects in the FANCD2 gene result in Fanconi anemia complementation group D2. This disorder affects all bone marrow elements resulting in anemia, leukopenia, and thrombopenia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.