Thermo Fisher Scientific 53BP1 Polyclonal Antibody
53BP1 단백질을 인식하는 Rabbit Polyclonal Antibody로 다양한 종에서 반응. Western blot, IHC, ICC/IF 등 여러 응용에 사용 가능. DNA 손상 반응 및 종양 억제 연구에 적합. 항원 친화 크로마토그래피로 정제된 고품질 시약.
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Thermo Fisher Scientific 53BP1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:1,000–1:5,000 | - |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:5,000 | 3 publications |
| Flow Cytometry (Flow) | 1.5 µg/mL | - |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| ChIP assay (ChIP) | Assay-Dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bat, Bovine, Dog, Fish, Goat, Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Epitope between residues 350–400 of human tumor protein p53 binding protein 1 (NP_005648.1, GeneID 7158) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_568422 |
Product Specific Information
- Human tested in WB, IHC-P, and ICC/IF
- Mouse and goat tested only in ICC/IF
- Suggested positive controls: U205, 293T, MO59K cell lysates, and MEF lysates
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and acts as a substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to double-strand break sites. It contributes to checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and maintains genomic stability through DNA repair.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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