Thermo Fisher Scientific ZEB1 Monoclonal Antibody (3G6), eBioscience
인간, 마우스, 랫트 ZEB1 단백질을 인식하는 3G6 클론 단일클론 항체입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. ZEB1 관련 EMT 및 유전자 발현 연구에 유용합니다.
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Thermo Fisher Scientific ZEB1 Monoclonal Antibody (3G6), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 4 publications |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 0.5 µg/mL | - |
| ChIP assay (ChIP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3G6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572969 |
Product Specific Information
The monoclonal antibody 3G6 recognizes human, mouse, and rat ZEB1. ZEB1 (zinc finger E-box binding homeobox 1), also known as Transcription Factor 8 (TCF8), Negative Regulator of IL2, and AREB6, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression.
It regulates expression of E-cadherin, BCL6, ATP1A1, and RCOR1 through binding to enhancer boxes (E-boxes), promoting epithelial-mesenchymal transition (EMT) and cancer invasion.
ZEB1 is expressed in differentiating tissues such as T cells and skeletal tissues, and mutations have been associated with corneal dystrophy.
Applications Reported:
This 3G6 antibody has been used for western blotting and IHC of formalin-fixed paraffin-embedded tissue sections.
Applications Tested:
Tested by IHC on formalin-fixed paraffin-embedded human tissue using high pH antigen retrieval. Recommended ≤10 µg/mL with titration for optimal performance.
Purity: Greater than 90%, determined by SDS-PAGE.
Aggregation: Less than 10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ZEB1 (Zinc finger E-box-binding homeobox 1) functions as a transcriptional repressor inhibiting IL-2 gene expression. It modulates ATP1A1 promoter activity depending on cDNA quantity and cell type.
ZEB1 represses E-cadherin promoters and induces EMT by recruiting SMARCA4/BRG1, represses BCL6 transcription via CTBP1, and regulates neuronal differentiation and RCOR1 transcription during neurogenesis.
Mutations in ZEB1 are associated with corneal dystrophies, including Fuchs endothelial dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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