Thermo Fisher Scientific TdT Monoclonal Antibody (7UNA8U6), eBioscience
Human, mouse, rat TdT 인식하는 단클론 항체로, Western blot 및 IHC(Paraffin)에 사용 가능. 0.5 mg/mL 액상 형태이며, PBS buffer(0.09% sodium azide) 포함. TdT는 림프구 발달 과정에서 N-뉴클레오타이드 추가에 관여하는 핵 내 DNA 중합효소.
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Thermo Fisher Scientific TdT Monoclonal Antibody (7UNA8U6), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7UNA8U6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572965 |
Product Specific Information
The monoclonal antibody 7UNA8U6 reacts with human, mouse, and rat TdT (terminal-deoxynucleotidyl transferase), a 60 kDa polymerase responsible for template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes.
Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. TdT plays a critical role in TCR and Ig gene rearrangement, increasing lymphocyte repertoire diversity through addition of N-nucleotides.
After positive selection, TdT expression decreases or becomes absent. TdT expression has been observed in lymphoma or lymphoblastic leukemia cells.
Applications Reported:
- Western blotting
- Immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections
Applications Tested:
- Immunohistochemistry (human and mouse tissue, low pH antigen retrieval): ≤10 µg/mL
- Western blot (mouse and rat cell lysate): ≤5 µg/mL
It is recommended to titrate the antibody for optimal performance.
Purity: >90%, determined by SDS-PAGE
Aggregation: <10%, determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase located in the cell nucleus that catalyzes polymerization of deoxynucleotides at 3′ hydroxyl ends of oligo- or polydeoxynucleotide initiators, functioning without a template.
TdT is a highly specific marker for diagnosis and classification of acute lymphoblastic lymphoma/leukemia. Determination of TdT expression is valuable for differentiating lymphoblastic lymphoma from Burkitt’s lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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