Thermo Fisher Scientific Globo H Monoclonal Antibody (VK9), eBioscience
인간 Globo H 항원을 인식하는 VK9 단클론 항체로, 다양한 암세포 표면의 항원 분석에 활용 가능. Western blot, IHC, Flow cytometry 등 다중 응용 가능. 고순도(>90%) 및 낮은 응집도(<10%)로 안정적 실험 재현성 제공. 연구용으로만 사용.
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Thermo Fisher Scientific Globo H Monoclonal Antibody (VK9), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | — |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | — |
| Flow Cytometry (Flow) | Assay-Dependent | Refer to publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG3, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VK9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with proprietary stabilizer |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2865397 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The VK9 monoclonal antibody recognizes human Globo H, a hexasaccharide derived from the pentasaccharide precursor SSEA3 (stage-specific embryonic antigen 3). Globo H belongs to a family of antigenic carbohydrates expressed on cancer cell surfaces as glycolipids and possibly glycoproteins.
Globo H is overexpressed in multiple carcinomas including breast, colon, ovarian, gastric, pancreatic, lung, and prostate cancers. Expression is found in more than half of ductal, lobular, and tubular breast carcinomas, but not in non-epithelial breast tumors. It is also expressed on cancer stem cells and human embryonic stem cells, with expression decreasing upon differentiation toward neural progenitor or endodermal lineage cells. In normal tissues, expression is limited to cells composing secretory borders.
Globo H is considered a potential immunotherapy target for various epithelial cancers.
Applications Reported:
This VK9 antibody has been reported for use in:
- Flow cytometric analysis
- Western blotting
- Immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections
- Microscopy
Applications Tested:
- Tested by IHC of formalin-fixed paraffin-embedded human tissue using high or low pH antigen retrieval at ≤5 µg/mL.
- Tested by Western blot of reduced MCF-7 cell lysate at ≤5 µg/mL.
- Recommended to titrate for optimal assay performance.
Target Information
Globo H is a hexasaccharide formed from the pentasaccharide precursor SSEA3. It is expressed on the surface of cancer cells and is overrepresented in many carcinomas. Expression is limited in normal tissues and is considered an important target for cancer immunotherapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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