Thermo Fisher Scientific Wnt1 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot contents and freeze at -20°C or below for extended storage.
• Avoid cycles of freezing and thawing.
• Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

This antibody reacts with human and mouse Wnt1 protein.
BLAST analysis suggests cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, and opossum (100% homology).
Partial cross-reactivity expected with chicken Wnt1 (91% sequence homology).
Cross-reactivity with other sources not determined.

Target Information

Wnt proteins are secreted glycoproteins that activate signal transduction pathways involved in tissue homeostasis, cell fate, proliferation, and self-renewal.
Canonical Wnt signaling involves Frizzled and LRP5/LRP6 receptors, activating the beta-catenin cascade.
Non-canonical signaling involves Frizzled and ROR2 receptors.
Deregulation of these pathways can lead to cancer.
Human Wnt-1 is a 370 amino acid precursor cleaved into a 343 amino acid (42 kDa) mature form.
Mature human Wnt-1 shares ~99% identity with mouse Wnt-1.
Wnt-1 is involved in embryonic development, carcinogenesis, and can induce phosphorylation and degradation of c-Myb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.