Thermo Fisher Scientific Wnt1 Polyclonal Antibody
Wnt1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western blot, ELISA, IP에 적합합니다. Human과 Mouse Wnt1에 반응하며, 다양한 종에서 교차반응 가능성이 있습니다. Affinity chromatography로 정제된 액상 제품으로 -20°C에서 보관합니다.
- 카탈로그번호
- 600-401-A37
- 판매단위
- pk
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1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Wnt1 Polyclonal Antibody
Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| ELISA | 1:25,000 |
| Immunoprecipitation (IP) | 1:1,500–1:6,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Marsupial, Mouse, Rhesus monkey, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from rabbit serum immunized with a synthetic peptide corresponding to an internal region of human Wnt1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody reacts with human and mouse Wnt1 protein.
BLAST analysis suggests cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, and opossum (100% homology).
Partial cross-reactivity expected with chicken Wnt1 (91% sequence homology).
Cross-reactivity with other sources not determined.
Target Information
Wnt proteins are secreted glycoproteins that activate signal transduction pathways involved in tissue homeostasis, cell fate, proliferation, and self-renewal.
Canonical Wnt signaling involves Frizzled and LRP5/LRP6 receptors, activating the beta-catenin cascade.
Non-canonical signaling involves Frizzled and ROR2 receptors.
Deregulation of these pathways can lead to cancer.
Human Wnt-1 is a 370 amino acid precursor cleaved into a 343 amino acid (42 kDa) mature form.
Mature human Wnt-1 shares ~99% identity with mouse Wnt-1.
Wnt-1 is involved in embryonic development, carcinogenesis, and can induce phosphorylation and degradation of c-Myb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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