Thermo Fisher Scientific Sipa1 Polyclonal Antibody
Western blot, IHC, ELISA 등 다양한 응용에 적합한 Sipa1 폴리클로날 항체. Rabbit IgG 기반으로 Human, Mouse, Rat 반응성. Affinity chromatography로 정제된 액상형 항체로 안정적이며 연구용으로 사용 가능.
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Thermo Fisher Scientific Sipa1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1.25–2.5 µg/mL |
| ELISA | 1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of mouse Sipa1. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.25 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for mouse Sipa1 protein. A BLAST analysis suggests cross-reactivity with Sipa1 from mouse, human, and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Sipa1 from other sources has not been determined.
Target Information
The product of this gene is a mitogen-induced GTPase activating protein. It exhibits specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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