Thermo Fisher Scientific Phospho-DARPP-32 (Thr34) Polyclonal Antibody
Phospho-DARPP-32 (Thr34) 항체로 인간, 마우스, 랫트 시료에서 인산화된 DARPP-32 단백질 검출에 사용. Western blot 및 IHC(P) 적용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체. 장기 보관 시 -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human DARPP-32 around the phosphorylation site of Threonine 34 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2553773 |
Product Specific Information
- Detects endogenous DARPP-32 protein at a molecular weight of 32 kDa.
- Purity is >95% by SDS-PAGE.
Target Information
DARPP-32 is a dopamine (DA) and AMP-regulated ~32 kDa phosphoprotein associated with dopaminoceptive neurons.
When phosphorylated at Thr34, DARPP-32 inhibits Protein Phosphatase I.
When phosphorylated at Thr75, it acts as an inhibitor of PKA.
Phosphorylation of DARPP-32 plays a critical role in dopaminergic neurotransmission and is implicated in the actions of alcohol, caffeine, and Prozac®.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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