Thermo Fisher Scientific Phospho-CPI-17 (Thr38) Polyclonal Antibody
Phospho-CPI-17 (Thr38) 단백질 인산화 부위를 인식하는 토끼 폴리클로날 항체. Western blot과 IHC(Paraffin)에 적합하며, 인간·마우스·랫트 반응성. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 단백질 인산화 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human CPI-17 around the phosphorylation site of Threonine 38 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553771 |
Product Specific Information
- Detects endogenous CPI-17 protein at approximately 22 kDa.
- Purity >95% by SDS-PAGE.
Target Information
CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphatase. It was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, dominantly expressed in smooth muscle.
Phosphorylation at Threonine 38 by PKC or Rho-kinase enhances inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N, possibly involved in calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid.
CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase (M110 kinase).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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