Thermo Fisher Scientific MEK1 C-Term Polyclonal Antibody, DyLight 680
DyLight 680 형광 표지된 MEK1 C-말단 폴리클로날 항체로 인간, 생쥐, 랫드에 반응합니다. Western blot과 ELISA에 적합하며 높은 특이성과 민감도를 제공합니다. 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1 µg/mL
ELISA
- Tested Dilution: 1:40,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-MEK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH. |
| Conjugate | DyLight™ 680 |
| Excitation / Emission Max | 676 / 705 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This DyLight™ 680 conjugated affinity purified antibody is directed against human MEK1 protein. Cross-reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform.
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation.
MAP2K1 is activated by a wide variety of growth factors and cytokines, and also by membrane-dependent depolarization and calcium influx.
Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is involved in signaling during stress response, apoptosis, and proliferative induction by cytokines.
Mutations in the gene can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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