Thermo Fisher Scientific ERK1 N-Term Polyclonal Antibody, DyLight 680
DyLight 680 형광 표지된 ERK1 N-말단 폴리클로날 항체로, Western blot 및 ELISA에 적합합니다. 사람, 생쥐, 랫트에 반응하며 ERK2와는 반응하지 않습니다. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the N-terminus of ERK1 conjugated to KLH |
| Conjugate | DyLight™ 680 |
| Excitation/Emission Max | 676/705 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This DyLight™ 680 conjugated affinity purified antibody is directed against human ERK1 protein. Cross-reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK2 isoform.
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that activates p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK signaling pathway. ERK1 is widely expressed and regulates meiosis, mitosis, and post-mitotic functions in differentiated cells. It is activated by various stimuli including growth factors, cytokines, viral infection, GPCR ligands, and transforming agents. Activated ERK1 translocates to the nucleus to phosphorylate nuclear targets, influencing proliferation, differentiation, and cell cycle progression. Alternative splicing generates multiple isoforms of ERK1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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