Thermo Fisher Scientific CD184 (CXCR4) Monoclonal Antibody (2B11), PE-eFluor 610, eBioscience

Thermo Fisher Scientific CD184 (CXCR4) Monoclonal Antibody (2B11), PE-eFluor 610, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1 µg/test
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Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 805
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Product Specific Information

The 2B11 monoclonal antibody reacts with mouse and human CXCR4 (CD184), also known as Fusin, LESTR, or HUMSTR. CXCR4 is a G-protein-coupled chemokine receptor that functions as a coreceptor for X4 HIV-1 entry into CD4+ cells and plays a role in lymphocyte migration. Its ligand is SDF1 and it is essential for B cell development and maintenance of granulocyte progenitors in bone marrow.

The 2B11 clone recognizes the amino terminus of CXCR4 and induces receptor internalization without blocking SDF1a.
This antibody is validated for flow cytometric analysis of mouse thymocytes and can be used at ≤1 µg/test (100 µL final volume). Optimal performance should be titrated empirically.

Fluorochrome Information:
PE-eFluor® 610 can be excited with 488–561 nm lasers and emits at 607 nm. Use a 610/20 bandpass filter (PE-Texas Red® equivalent). Protect from light due to photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be verified experimentally.

Excitation/Emission:
488–561 nm / 607 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a G-protein-coupled chemokine receptor with seven membrane-spanning domains. It serves as a co-receptor for HIV-1 and HIV-2 entry into CD4+ cells via gp120-mediated fusion. CXCR4 binds CXCL12/SDF-1, mediating calcium signaling and MAPK activation, and also acts as a receptor for extracellular ubiquitin. It plays key roles in hematopoiesis, cardiac and vascular development, and CNS neuronal survival. CXCR4 binding to bacterial LPS mediates inflammatory responses, including TNF secretion. Antibodies against CXCR4 can block HIV fusion and infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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