Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), Super Bright 436, eBioscience
마우스 CD1d 인식용 Rat IgG2b 단일클론 항체로, Super Bright 436 형광체 결합. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저로 여기되어 436 nm에서 방출. 고순도 친화크로마토그래피 정제 및 안정적 PBS 버퍼 보존.
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Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), Super Bright 436, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test
Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1B1 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744761 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- APC
- Biotin
- Brilliant Violet 480
- Brilliant Violet 711
- eFluor 450
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685 / 700 / 755
- NovaFluor Yellow 610 / 660 / 700 / 755
- PE
- PerCP-eFluor 710
- Super Bright 600 / 645
- Request custom conjugation
Product Specific Information
The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein structurally homologous to MHC class I molecules. CD1d associates with β2-microglobulin and functions similarly to MHC class II. The 1B1 antibody detects CD1d at varying levels on mouse leukocytes and recognizes β2m-associated CD1d.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; ≤ 0.5 µg/test in 100 µL final volume. Optimal titration recommended.
Fluorochrome Information:
Super Bright 436 is excited at 405 nm (violet laser) and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent.
When using multiple Super Bright conjugates, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Laser: Violet (405 nm)
Emission: 436 nm
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD1d is a heavy chain associated with β2-microglobulin on cortical thymocytes. It is a unique group 2 member of the CD1 family, structurally related to MHC proteins, forming heterodimers with β2-microglobulin. CD1 molecules present lipid and glycolipid antigens to T cells. The human genome contains five CD1 family genes on chromosome 1, differing in localization and ligand specificity. CD1d localizes to late endosomes and lysosomes via a cytoplasmic tail motif. Diseases associated include Mycobacterium Malmoense and autoimmune disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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