Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), PE-eFluor 610, eBioscience

Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), PE-eFluor 610, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 480, 605, 650, 786
• eFluor 450, 506
• FITC
• Functional Grade
• PE, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes.

Two ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC), interact with PD-1 to mediate inhibitory signals. J43 inhibits the binding of mouse PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells. In vivo administration of J43 enhances contact hypersensitivity and exacerbates acute GVHD, EAE, and NOD diabetes similarly to PD-1-deficient cells.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of stimulated mouse splenocytes at ≤1 µg/test.
A test is defined as staining a cell sample (100 µL final volume). Cell count may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.

Fluorochrome Information:
PE-eFluor® 610 can be excited with 488–561 nm lasers and emits at 607 nm. Recommended filter: 610/20 band pass (PE-Texas Red® equivalent). Protect from light to avoid photo-oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333). Clone-specific performance should be verified empirically.

Excitation / Emission / Laser:

• Excitation: 488–561 nm
• Emission: 607 nm
• Laser: Blue, Green, Yellow-Green

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

T-cell activation is self-limited through inhibitory receptors such as PD-1 (PDCD-1), which binds PD-L1 and PD-L2 ligands on antigen-presenting cells. PD-1 engagement inhibits immune activation in the absence of danger signals (e.g., LPS). PD-1-deficient mice exhibit hyperactivated immune systems and autoimmune diseases. PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures.

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