Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker) Monoclonal Antibody (EGP40/1373)
Ep-CAM/CD326 단백질의 세포외 도메인을 인식하는 마우스 단클론 항체로, 인간·개·고양이 반응성. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. Protein A/G 정제, PBS/BSA 완충액 보존, 4°C 보관.
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Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) Monoclonal Antibody (EGP40/1373)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10⁶ cells |
| ELISA | Assay-dependent |
| Peptide Array | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Cat, Human |
| Host / Isotype | Mouse / IgG, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EGP40/1373 |
| Immunogen | Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100–224) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a Ca²⁺-independent homotypic adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclins A and E.
Ep-CAM-mediated adhesion negatively regulates cadherin-mediated adhesion, affecting epithelial differentiation and growth. Overexpression is associated with increased epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors.
Ep-CAM is localized to the basolateral surface of most simple epithelia and many carcinoma types, and can help distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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