Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker) Monoclonal Antibody (EGP40/1384)
Ep-CAM/CD326 단백질의 세포외 도메인을 인식하는 마우스 단클론 항체로, 인간·개·고양이 시료에 반응합니다. IHC, ICC/IF, Flow Cytometry, ELISA 등 다양한 응용에 적합하며, 단백질 A/G로 정제된 액상 형태입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
| ELISA | Assay-dependent |
| Peptide Array | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Cat, Human |
| Host / Isotype | Mouse / IgG, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EGP40/1384 |
| Immunogen | Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100–224) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
Ep-CAM (Epithelial Cell Adhesion Molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent homotypic cell adhesion molecule and influences cell cycle, proliferation, and metabolism through induction of proto-oncogene c-myc and cyclins A and E. Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is associated with increased epithelial proliferation and is a marker for tumors of epithelial origin. It is expressed on basolateral surfaces of simple epithelia and many carcinoma types and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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