Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker) Monoclonal Antibody (EGP40/1373)
Ep-CAM/CD326 단백질의 세포 부착 및 증식 관련 연구용 항체. 인간, 개, 고양이에 반응하며 다양한 응용(Flow, WB, IHC 등)에 적합. 단클론성 Mouse IgG, Protein A/G 정제, PBS 용액 형태로 제공. 연구용 전용 제품.
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Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) Monoclonal Antibody (EGP40/1373)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
| ELISA | Assay-dependent |
| Peptide Array | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Cat, Human |
| Host / Isotype | Mouse / IgG, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EGP40/1373 |
| Immunogen | Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100–224) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial cells. It functions as a homotypic, calcium-independent adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing c-myc and cyclins A/E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is associated with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors.
Ep-CAM is found on the basolateral surface of most simple epithelia and many carcinoma types, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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