Thermo Fisher Scientific Ep-CAM/CD326 Monoclonal Antibody (EGP40, 1372)
Ep-CAM/CD326 단클론 항체(EGP40, 1372)는 인간, 개, 고양이에 반응하며 다양한 응용(WB, IHC, ICC, Flow 등)에 적합합니다. Recombinant EpCAM 항원을 이용해 제작된 Mouse IgG1 항체로, 단백질 A/G로 정제된 액상형 제품입니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific Ep-CAM/CD326 Monoclonal Antibody (EGP40, 1372)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Cat, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EGP40, 1372 |
| Immunogen | Recombinant human EpCAM fragment from extracellular domain (around aa 100–224) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature (RT).
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at RT for 20 min.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues.
It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism via induction of proto-oncogene c-myc and cyclins A/E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth.
Ep-CAM is highly expressed in human carcinomas and serves as a marker for epithelial lineage tumors. It is located on the baso-lateral surface of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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