Thermo Fisher Scientific Thrombopoietin Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Thrombopoietin 폴리클로날 항체로, Western Blot, ELISA, Immunostaining에 사용 가능. 인간 TPO에 특이적이며, 고순도 재조합 단백질로부터 면역화된 토끼 혈청에서 생산. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 민감도 제공.
- 카탈로그번호
- 500-P49BT-500UG
- 판매단위
- pk
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Applications
Western Blot (WB)
- Tested Dilution: 0.1–0.2 µg/mL
- Detection limit: 1.5–3.0 ng/lane (reducing or non-reducing conditions)
ELISA
- Tested Dilution: 0.25–1.0 µg/mL
- Sandwich ELISA: Detects 0.2–0.4 ng/well of Recombinant hTPO
- Recommended use with PeproTech Polyclonal Anti-Human TPO (500-P49) as capture antibody
Immunostaining (IS)
- Tested Dilution: Not specified
- 1 publication available
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 18.6 kDa Recombinant Human TPO |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
AA Sequence of recombinant protein:
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TPO. Anti-Human TPO-specific antibody was purified by affinity chromatography and then biotinylated.
Western Blot:
To detect hTPO by Western Blot analysis, use at 0.1–0.2 µg/mL. Detection limit for Recombinant hTPO is 1.5–3.0 ng/lane.
Sandwich ELISA:
To detect hTPO by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TPO (500-P49) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hTPO.
Packaging:
500-P49BT-1MG is provided as 2 × 500 µg.
Target Information
Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) regulates platelet production by acting on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor MPL (CD110) activates JAK/STAT, MAPK, and PI3K/AKT signaling pathways, leading to megakaryocyte proliferation and differentiation. TPO is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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