Thermo Fisher Scientific Thrombopoietin Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL
• Detection limit: 1.5–3.0 ng/lane (reducing or non-reducing conditions)

ELISA

• Tested Dilution: 0.25–1.0 µg/mL
• Sandwich ELISA: Detects 0.2–0.4 ng/well of Recombinant hTPO
• Recommended use with PeproTech Polyclonal Anti-Human TPO (500-P49) as capture antibody

Immunostaining (IS)

• Tested Dilution: Not specified
• 1 publication available

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TPO. Anti-Human TPO-specific antibody was purified by affinity chromatography and then biotinylated.

Western Blot:
To detect hTPO by Western Blot analysis, use at 0.1–0.2 µg/mL. Detection limit for Recombinant hTPO is 1.5–3.0 ng/lane.

Sandwich ELISA:
To detect hTPO by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TPO (500-P49) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hTPO.

Packaging:
500-P49BT-1MG is provided as 2 × 500 µg.

Target Information

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) regulates platelet production by acting on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor MPL (CD110) activates JAK/STAT, MAPK, and PI3K/AKT signaling pathways, leading to megakaryocyte proliferation and differentiation. TPO is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.