Thermo Fisher Scientific SCF Polyclonal Antibody, PeproTech
Human SCF 인식 Goat Polyclonal 항체로 Western blot, ELISA, Immunostaining 등에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체이며, Recombinant Human SCF 검출에 높은 민감도 제공. 연구용으로만 사용.
- 카탈로그번호
- 500-P48G-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific SCF Polyclonal Antibody, PeproTech
Thermo Fisher Scientific SCF Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | - |
| Neutralization (Neu) | - | View 1 publication |
| Immunostaining (IS) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human SCF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930575 |
Product Specific Information
AA Sequence of recombinant protein:
MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human SCF. Anti-Human SCF-specific antibody was purified by affinity chromatography employing an immobilized Human SCF matrix.
Sandwich ELISA:
To detect Human SCF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human SCF (500-P48GBt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human SCF.
Western Blot:
To detect hSCF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hSCF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and activation of signaling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways.
SCF expression induces mast cell survival and the release of histamine, pro-inflammatory cytokines, and chemokines. Inhibition of the SCF/c-Kit pathway decreases histamine levels, mast cell and eosinophil infiltration, IL-4 production, and airway hyperresponsiveness, suggesting this pathway may be a potential therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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