Thermo Fisher Scientific SCF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human SCF. Anti-Human SCF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hSCF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SCF (500-P48G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hSCF.

Western Blot:
To detect hSCF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hSCF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P48GBT-1MG will be provided as 2 × 500 µg.

Target Information

Stem Cell Factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF to the c-Kit receptor leads to receptor homodimerization and activation of signaling pathways such as PI-3, PLC-γ, Jak/STAT, and MAP kinase. SCF expression promotes mast cell survival and the release of histamine, pro-inflammatory cytokines, and chemokines. Inhibition of the SCF/c-Kit pathway reduces histamine levels, mast cell and eosinophil infiltration, IL-4 production, and airway hyperresponsiveness, suggesting potential therapeutic relevance in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.