Thermo Fisher Scientific CD137 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MERTRSLQDP CSNCPAGTFC DNNRNQICSP CPPNSFSSAG GQRTCDICRQ CKGVFRTRKE CSSTSNAECD CTPGFHCLGA GCSMCEQDCK QGQELTKKGC KNCCFGTFND QKRGICRPWT NCSLDGKSVL VNGTKERDVV CGPSPADLSP GASSVTPPAP AREPGHS.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human 4-1BB Receptor. Anti-Human 4-1BB Receptor-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect h4-1BB Receptor by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human 4-1BB Receptor (500-P167G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant h4-1BB Receptor.

Western Blot:
To detect h4-1BB Receptor by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant h4-1BB Receptor is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P167GBT-1MG is provided as 2 × 500 µg.

Target Information

CD137 (also known as TNFRSF9 or 4-1BB) is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells.
CD137 signaling supports the survival of activated and memory CD8+ T cells, promotes clonal expansion, and modulates immune responses by regulating cytokine production and monocyte survival. Soluble CD137 may act as a negative regulator of immune activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.