Thermo Fisher Scientific CD137 Polyclonal Antibody, PeproTech
Human CD137(4-1BB) 단백질을 인식하는 염소 유래 Polyclonal 항체로, Western Blot과 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 내 동결건조 형태로 제공됩니다. 활성화된 T세포 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human 4-1BB Receptor (CD137, UniProt ID: Q07011-1, amino acids 19–184) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929594 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MERTRSLQDP CSNCPAGTFC DNNRNQICSP CPPNSFSSAG GQRTCDICRQ CKGVFRTRKE CSSTSNAECD CTPGFHCLGA GCSMCEQDCK QGQELTKKGC KNCCFGTFND QKRGICRPWT NCSLDGKSVL VNGTKERDVV CGPSPADLSP GASSVTPPAP AREPGHS
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human 4-1BB Receptor. Anti-Human 4-1BB Receptor-specific antibody was purified by affinity chromatography employing an immobilized Human 4-1BB Receptor matrix.
Sandwich ELISA:
To detect Human 4-1BB Receptor by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human 4-1BB Receptor (500-P167GBt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human 4-1BB Receptor.
Western Blot:
To detect Human 4-1BB Receptor by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human 4-1BB Receptor is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling maintains survival of activated and CD8+ memory T cells, promotes clonal expansion of T cells, and suppresses myelopoiesis and dendritic cell development. Triggered CD137 induces cytokine release regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanisms for certain immune responses.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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