Thermo Fisher Scientific TNFR2 (soluble) Polyclonal Antibody, PeproTech
인간 TNFR2(sTNF Receptor Type II)에 특이적인 Rabbit Polyclonal 항체로, Western blot, ELISA, ICC/IF 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, Lyophilized 형태로 제공. 연구용으로만 사용.
- 카탈로그번호
- 500-P168-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TNFR2 (soluble) Polyclonal Antibody, PeproTech
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | - |
| Miscellaneous PubMed | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human sTNF Receptor Type II |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929600 |
Product Specific Information
AA Sequence of recombinant protein:
MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type II. Anti-Human sTNF Receptor Type II-specific antibody was purified by affinity chromatography employing an immobilized Human sTNF Receptor Type II matrix.
Sandwich ELISA:
To detect Human sTNF Receptor Type II by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sTNF Receptor Type II (500-P168Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human sTNF Receptor Type II.
Western Blot:
To detect Human sTNF Receptor Type II by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human sTNF Receptor Type II is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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