Thermo Fisher Scientific TNFR2 (soluble) Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal 항체로, Human TNFR2(sTNFR2)를 인식합니다. Western blot과 ELISA에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 동결건조 형태로 제공되며, -20°C에서 보관합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 18.9 kDa Recombinant Human TNF Receptor Type II |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type II. Anti-Human sTNF Receptor Type II-specific antibody was purified by affinity chromatography and subsequently biotinylated.
Sandwich ELISA:
To detect hsTNF Receptor II by sandwich ELISA (using 100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTNF Receptor II (500-P168) as a capture antibody, enables detection of at least 0.2–0.4 ng/well of Recombinant hsTNF Receptor II.
Western Blot:
To detect hsTNF Receptor II by Western Blot analysis, use 0.1–0.2 µg/mL of this antibody. When used with compatible secondary reagents, the detection limit for Recombinant hsTNF Receptor II is 1.5–3.0 ng/lane under both reducing and non-reducing conditions.
Packaging:
500-P168BT-1MG is provided as 2 × 500 µg.
Target Information
TNFR2 (Tumor Necrosis Factor Receptor Superfamily Member 1B)
TNFR2 is a receptor with high affinity for TNFSF2/TNF-alpha and lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates many metabolic effects of TNF-alpha.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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