Thermo Fisher Scientific TNFR2 (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type II. Anti-Human sTNF Receptor Type II-specific antibody was purified by affinity chromatography and subsequently biotinylated.

Sandwich ELISA:
To detect hsTNF Receptor II by sandwich ELISA (using 100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTNF Receptor II (500-P168) as a capture antibody, enables detection of at least 0.2–0.4 ng/well of Recombinant hsTNF Receptor II.

Western Blot:
To detect hsTNF Receptor II by Western Blot analysis, use 0.1–0.2 µg/mL of this antibody. When used with compatible secondary reagents, the detection limit for Recombinant hsTNF Receptor II is 1.5–3.0 ng/lane under both reducing and non-reducing conditions.

Packaging:
500-P168BT-1MG is provided as 2 × 500 µg.

Target Information

TNFR2 (Tumor Necrosis Factor Receptor Superfamily Member 1B)
TNFR2 is a receptor with high affinity for TNFSF2/TNF-alpha and lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates many metabolic effects of TNF-alpha.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.