Thermo Fisher Scientific TRIB1 Monoclonal Antibody (OTI1B11), TrueMAB
TRIB1 단백질을 인식하는 Mouse IgG1 단일클론 항체로 Western blot에 최적화됨. Human 시료 반응성 확인. Lyophilized 형태로 제공되며, PBS buffer 및 trehalose 포함. 재구성 후 결합 실험에 적합하며 연구용으로만 사용 가능.
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Applications
- Western Blot (WB)
Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B11 |
| Immunogen | Full length human recombinant protein of human TRIB1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
TRIB1 (also known as gprotein coupled receptor induced, skip1, gig2, c8fwATP) is involved in protein binding, protein kinase inhibitor activity, and serine/threonine kinase activities.
Overexpression of TRIB1 in HeLa cells represses IL8 promoter activity by inhibiting AP1. It also inhibits Ras-driven and MEKK1-mediated AP1 activation. ERK activation is enhanced by TRIB1.
Interactions include MEK1 with TRIB1 and TRIB3, and MKK4 specifically with TRIB1. Cotransfection of MKK4 stabilizes TRIB1 through TRIB-MAPKK interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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