Thermo Fisher Scientific PTGS2 Monoclonal Antibody (OTI1B4), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a key step in prostanoid synthesis, including inflammatory prostaglandins.
This conversion involves two reactions:

1. Cyclooxygenase (COX) reaction converting arachidonate to prostaglandin G2 (PGG2)
2. Peroxidase reaction reducing PGG2 to PGH2

COX2 is constitutively expressed in some tissues (endothelium, kidney, brain) and up-regulated under pathological conditions such as cancer and inflammation, unlike PTGS1 which is expressed ubiquitously.
Up-regulation is associated with increased cell adhesion, phenotypic changes, resistance to apoptosis, and tumor angiogenesis.
In cancer cells, COX2 drives prostaglandin E2 (PGE2) production, influencing motility, proliferation, and apoptosis resistance.
COX2 is naturally inhibited by calcitriol (active form of Vitamin D).
Glucocorticoids repress PTGS2 gene activity by interfering with transcription initiation and elongation.
COX2 is a target of NSAIDs (e.g., aspirin) that reduce pain and inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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