Thermo Fisher Scientific PAX5 Monoclonal Antibody (1H9), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

3-5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Flow Cytometry (Flow)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-Dependent

-

ChIP assay (ChIP)

2.5 µg/10^6 cells

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1H9

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• PE
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865519

Product Specific Information

Description: The monoclonal antibody 1H9 recognizes both mouse and human Pax5. Pax5, also known as BSAP (B cell specific activator protein), is a member of the paired box (pax) family of transcription factors. Pax5 is the only member of the pax family of transcription factors that is expressed in hematopoietic cells. During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development at the time of B cell commitment and is maintained throughout most subsequent stages. Suppression of Pax5 is essential for expression of Blimp-1 and the terminal differentiation of plasma cells. In the spleen, expression of Pax5 is higher in marginal zone B cells (B220+ CD21high CD23low) than in other B cells, especially the transition 1 stage (B220+ CD21- CD23-). In addition to its role in B cell development, Pax5 also affects VH-DJH heavy chain recombination as well as influencing the expression of many other B and non-B cell related proteins. Pax5 expression is correlated with many neoplasms. In diffuse large B cell lymphomas (DLBCL) and non-Hodgkin lymphomas Pax5 is often mutated while in B-cell ALL, expression levels are high. Additionally, translocation with elastin, IGH, ETV6, FOXP1, and EVI3 have been identified.

eBioscience recommends that the Foxp3 Buffers (Product # 00-5521) be used for optimal results when using this antibody for intracellular staining and flow cytometric analysis.

Applications Reported: This 1H9 antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections.

Applications Tested: This 1H9 antibody has been tested by western blot analysis. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.