Thermo Fisher Scientific PAX5 Monoclonal Antibody (1H9), eBioscience
PAX5 단백질을 인식하는 Rat 모노클로날 항체로, Human 및 Mouse 시료에 반응합니다. Western blot, IHC, ICC, ChIP 등 다양한 응용에 사용 가능하며, Affinity chromatography로 정제된 고순도 항체입니다. B세포 발달 및 관련 종양 연구에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 3–5 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
| Flow Cytometry (Flow) | - | View 2 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| ChIP assay (ChIP) | 2.5 µg/10⁶ cells | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865519 |
Additional Formats
Product Specific Information
The monoclonal antibody 1H9 recognizes both mouse and human Pax5. Pax5, also known as BSAP (B cell specific activator protein), is a member of the paired box (pax) family of transcription factors. It is the only member expressed in hematopoietic cells.
During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development and maintained through most stages. Suppression of Pax5 is essential for Blimp-1 expression and plasma cell differentiation.
In the spleen, Pax5 expression is higher in marginal zone B cells (B220⁺ CD21^high CD23^low) than in other B cells. Pax5 also influences VH-DJH recombination and the expression of various B and non-B cell proteins. Pax5 expression is correlated with many neoplasms, including DLBCL, non-Hodgkin lymphoma, and B-cell ALL. Various translocations (elastin, IGH, ETV6, FOXP1, EVI3) have been identified.
Recommended Reagents:
For optimal intracellular staining and flow cytometric analysis, eBioscience recommends the Foxp3 Buffers (Product #00-5521).
Applications Reported:
This 1H9 antibody has been reported for use in immunoprecipitation, western blotting (WB), and immunohistology of frozen tissue sections.
Applications Tested:
Tested by western blot analysis. Recommended ≤5 µg/mL. Optimize dilution for each assay.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
PAX5 is a transcription factor of the paired box (PAX) family, crucial in early development. It acts as a B-cell lineage-specific activator protein (BSAP), expressed in early but not late stages of B-cell differentiation. Expression is also detected in developing CNS and testis, suggesting a role in neural development and spermatogenesis. Mutations in PAX5 are associated with acute lymphoblastic leukemia.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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