Thermo Fisher Scientific CD4 Monoclonal Antibody (OKT4 (OKT-4)), Alexa Fluor 700, eBioscience
휴먼 CD4 항원을 인식하는 OKT4 모노클론 항체로, Alexa Fluor 700 형광체가 결합된 제품입니다. Flow Cytometry에 최적화되어 있으며, HIV 연구 및 T세포 분석에 활용됩니다. 고순도 정제, 안정적 형광 특성, 4°C 암소 보관 권장.
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Thermo Fisher Scientific CD4 Monoclonal Antibody (OKT4 (OKT-4)), Alexa Fluor 700, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 32 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Hamster, Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT4 (OKT-4) |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10718539 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- Brilliant Violet 650
- Brilliant Violet 786
- eFluor 450
- eFluor 660
- FITC
- Functional Grade
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Yellow 660
- PE
- PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
Description:
The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by thymocytes, T-helper cells, and monocytes. CD4 serves as a receptor for HIV. The OKT4 antibody recognizes a distinct epitope from RPA-T4 and does not cross-block its binding.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.125 µg) per test in a final volume of 100 µL. Cell number can range from 10⁵ to 10⁸ cells/test.
Fluorochrome Information:
Alexa Fluor™ 700 emits at 723 nm and can be excited with a red laser (633 nm). Recommended optical setup: 685 LP mirror and 710/20 filter.
- Excitation: 633–647 nm
- Emission: 723 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD4 antigen is involved in MHC class II recognition and functions as a co-receptor for HIV. Primarily expressed in T-helper cells, CD4 is also found in monocytes, macrophages, and dendritic cells. Tissue-level expression includes thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut. CD4 assists in T-cell activation through association with the T-cell receptor complex and Lck kinase, and may mediate neuronal damage in infectious or immune-mediated CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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