Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Alexa Fluor 700, eBioscience
Alexa Fluor 700으로 표지된 GK1.5 클론 CD4 단일클론 항체로, 마우스 CD4 분자를 인식합니다. Flow cytometry 및 다양한 면역분석(IHC, ICC/IF)에 적합하며, 고순도의 액상 형태로 제공됩니다. 633 nm 적색 레이저에 의해 여기되며, 723 nm에서 방출됩니다.
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Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Alexa Fluor 700, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 18 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 4 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 195 publications |
| Functional Assay (Functional) | - | View 3 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 700 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GK1.5 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_494001 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 661 / 737
- Brilliant Violet 480 / 605
- eFluor 450 / 506 / 660
- FITC
- Functional Grade
- NovaFluor Blue / Red / Violet / Yellow series
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation available
Product Specific Information
The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays a crucial role in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse thymocyte and splenocyte suspensions. Recommended ≤ 0.125 µg per test.
A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume. Cell number may range from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.
Fluorochrome Information:
Alexa Fluor® 700 emits at 723 nm and is excited with the red laser (633 nm). Most instruments require a 685 LP mirror and 710/20 filter.
- Excitation: 633–647 nm
- Emission: 723 nm
- Laser: Red
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. CD4 is mainly expressed on T helper cells but also found on monocytes, macrophages, and dendritic cells. Tissue-level expression includes thymus, lymph nodes, tonsils, spleen, and certain brain and gut regions. CD4 functions to initiate or augment early T-cell activation via association with the T-cell receptor complex and protein tyrosine kinase Lck. It may also mediate neuronal damage in infectious and immune-mediated CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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