Thermo Fisher Scientific Phospho-p130Cas (Tyr410) Polyclonal Antibody
p130Cas 단백질의 Tyr410 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P)에서 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer로 제공. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human p130Cas around the phosphorylation site of Tyrosine 410 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553680 |
Product Specific Information
This antibody detects endogenous p130Cas protein at a molecular weight of approximately 130 kDa.
Purity is greater than 95% by SDS-PAGE.
Target Information
p130Cas (Crk-associated substrate) is a common cellular target of phosphorylation signaling via v-Crk and v-Src oncoproteins.
It contains a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich region.
p130Cas is involved in various biological processes including:
- Cell adhesion and migration
- Growth factor stimulation
- Cytokine receptor engagement
- Bacterial infection
Physiological roles include cardiovascular development, actin filament assembly, and Src-induced cell transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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