Thermo Fisher Scientific Phospho-alpha Adducin (Thr445) Polyclonal Antibody
인산화된 alpha Adducin(Thr445)을 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(P) 분석에 적합하며, 인간, 마우스, 랫트 반응성. 고순도(>95%) 항원 친화 크로마토그래피 정제, 액상 형태로 제공.
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Thermo Fisher Scientific Phospho-alpha Adducin (Thr445) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Adducin alpha around the phosphorylation site of Threonine 445 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553686 |
Product Specific Information
- Detects endogenous protein at a molecular weight of 81 kDa.
- Purity is >95% by SDS-PAGE.
Target Information
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma).
Adducin is a heterodimeric protein composed of related subunits produced from distinct genes but sharing similar structure.
Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region.
Alpha- and gamma-adducins are ubiquitously expressed, whereas beta-adducin is highly expressed in brain and hematopoietic tissues.
Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C.
Alternative splicing results in multiple variants encoding distinct isoforms, not all of which have been fully described.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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