Thermo Fisher Scientific Phospho-POLR2A (Ser1619) Polyclonal Antibody
POLR2A Ser1619 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(Paraffin)에서 검증됨. 고순도(>95%) 항원 친화 크로마토그래피 정제. Human, Mouse, Rat 반응성. 연구용으로 RNA Polymerase II 기능 분석에 적합.
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Thermo Fisher Scientific Phospho-POLR2A (Ser1619) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Rpb1 around the phosphorylation site of Serine 1619 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553682 |
Product Specific Information
- Detects endogenous protein at molecular weights of 217 and 240 kDa.
- Purity >95% by SDS-PAGE.
Target Information
DNA-directed RNA polymerase II subunit RPB1 (POLR2A) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates.
POLR2A is the largest subunit and a catalytic component of RNA polymerase II, synthesizing mRNA precursors and non-coding RNAs.
It forms the polymerase active center with the second largest subunit and is central to the basal transcription machinery.
RPB1 contributes to the core element with the central cleft, clamp, and jaws that engage the DNA template.
Phosphorylation of the C-terminal domain (CTD) regulates transcription initiation, elongation, and termination, as well as mRNA processing.
Gene expression levels are influenced by the balance between methylation and acetylation of CTD lysines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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