Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), Alexa Fluor 700, eBioscience
인간 TNF alpha를 인식하는 중화성 단클론 항체로, Alexa Fluor 700으로 표지되어 있습니다. 유세포분석(Flow Cytometry)과 면역조직화학(IHC)에 사용 가능하며, 633 nm 레이저로 여기됩니다. 4°C 암소 보관, 동결 금지. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 28 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MAb11 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10670754 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 660
- APC
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 650
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
Description:
The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4+ T cells. TNF alpha has cytolytic activity against tumor cells and plays a critical role in immune regulation. It can form dimers and trimers and also exists as a 26 kDa membrane-bound form. The MAb11 antibody is a neutralizing antibody.
Applications Reported:
Reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00) and corresponding protocol. Recommended usage is 5 µL (0.25 µg) per test (100 µL final volume). Cell number per test may range from 10⁵ to 10⁸ depending on experimental conditions.
Fluorochrome Information:
Alexa Fluor® 700 emits at 723 nm and is excited with the red laser (633 nm). Recommended instrument setup includes a 685 LP mirror and 710/20 filter.
- Excitation: 633–647 nm
- Emission: 723 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune response, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It exists as multimers but shows a 17 kDa band under non-reducing SDS-PAGE conditions.
Knockout studies in mice indicate neuroprotective roles and tumor necrosis activity. TNF alpha contributes to immune responses against infections and tumor cytotoxicity, often synergistic with interferon-gamma. It is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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